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Pharmaceutical cocrystals are a promising technology that can be used to improve the solubility of poor aqueous compounds. The objective of this study was to systematically investigate the solubility of myricetin (MYR) cocrystals, including their kinetic solubility, thermodynamic solubility, and intrinsic dissolution rate (IDR). The effects of pH, surfactant, ion concentration, and coformers on the cocrystal solubility were evaluated. Furthermore, single crystal structures of MYR, myricetin-isonicotinamide (MYR-INM) and myricetin-caffeine (MYR-CAF) cocrystals were analyzed to discuss the possible reasons for the enhancement of cocrystal solubility from the perspective of the spatial structure. The results indicated that the kinetic solubility of MYR cocrystals was modulated by pH and cocrystal coformer (CCF) ionization in buffer solution, while it primarily depended on the CCF solubility in pure water. In addition, the solubility of MYR cocrystals was increased in a concentration dependent fashion by the surfactant or ion concentration. The thermodynamic solubility of MYR-INM (1:3) cocrystals decreased with the increases of the pH value of the dissolution media. The IDR of MYR cocrystals was faster than that of MYR in the same medium and extremely fast in pH 4.5 buffer. The improved solubility of MYR cocrystals was probably related to the alternate arrangements of MYR and INM/CAF molecules and increased intermolecular distance. The present study provides some references to investigate the solubility behavior of pharmaceutical cocrystals.
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OBJECTIVE:To provide reference for the further research and development of bamboo juice. METHODS:Comb-ing the ancient and modern literatures,the processing historical evolution,origins,technology,chemical ingredients,quality stan-dards and efficacy were analyzed,the problems were found and solutions were put forward. RESULTS:Bamboo juice's processing can be traced back to the Liang Dynasty,still being used today with light bamboo juice. And now,varieties of bamboos(Phyllo-stachys glauca,Phyllostachys viridiglaucescens,Pleioblastus amarus,and Neosino calamus,etc.) were processed into medicine. The research for the chemical ingredients was mainly for the changes of bamboo juice with different varieties and amino acids,phe-nols,organic acids and inorganic elements in bamboo juice with different technologies,and there was no comprehensive study on the material basis of bamboo juice. Its quality standards must be improved. Although there was comparative study for the traditional processing technology and modern technology in chemical ingredients,the study did not compare the efficacy of its material founda-tions. The research for the efficacy of bamboo juice only involved relieving cough and resolving phlegm,and its relieving convul-sion,resuscitation,calming diabetes and other pharmacological effects haven't been reported. CONCLUSIONS:In the future, in-dept research can be carried out from the aspects of standardizing the name,base source and processing technology parameters, improving quality standards,strengthening basic research of effective substances,and improving traditional technology and equip-ment level.
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OBJECTIVE:To provide reference for the further research and development of bamboo juice. METHODS:Comb-ing the ancient and modern literatures,the processing historical evolution,origins,technology,chemical ingredients,quality stan-dards and efficacy were analyzed,the problems were found and solutions were put forward. RESULTS:Bamboo juice's processing can be traced back to the Liang Dynasty,still being used today with light bamboo juice. And now,varieties of bamboos(Phyllo-stachys glauca,Phyllostachys viridiglaucescens,Pleioblastus amarus,and Neosino calamus,etc.) were processed into medicine. The research for the chemical ingredients was mainly for the changes of bamboo juice with different varieties and amino acids,phe-nols,organic acids and inorganic elements in bamboo juice with different technologies,and there was no comprehensive study on the material basis of bamboo juice. Its quality standards must be improved. Although there was comparative study for the traditional processing technology and modern technology in chemical ingredients,the study did not compare the efficacy of its material founda-tions. The research for the efficacy of bamboo juice only involved relieving cough and resolving phlegm,and its relieving convul-sion,resuscitation,calming diabetes and other pharmacological effects haven't been reported. CONCLUSIONS:In the future, in-dept research can be carried out from the aspects of standardizing the name,base source and processing technology parameters, improving quality standards,strengthening basic research of effective substances,and improving traditional technology and equip-ment level.
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Objective To investigate the expression of NLRP3 in different time point of HIBD neonatal rats and to search for critical time points and alleviate HIBD dysfunction.Methods 96 newborn rats of 7 days old were randomly divided into HIBD group(n=48) and Sham operation group(n=48).HIBD model was prepared by referring to Rice method.Brain tissue was taken after 6 h,24 h,72 h,7 d.Brain injury was detected by HE stain.The expression and distribution of NLRP3 and Caspase-1 were detected by immune fluorescence and Western blot,and IL-1β and IL-18 were detected by ELISA.Results HE staining and immunofluorescence showed that NLRP3 protein (HIBD group (0.63±0.07),Sham group(0.43±0.04)) was increased significantly since 6 h in HIBD group,and its downstream protein Caspase-1,IL-1β and IL-18 were successive activated.The results showed IL-1β (HIBD group(732.28± 108.42)pg/ml,Sham group(584.58± 36.35) pg/ml) was increased significantly since 6 h in HIBD group;Caspase-1 (HIBD group(0.67±0.09),Sham group(0.30±0.05)),IL-18 (HIBD group(683.84±31.83) pg/ml,Sham group(571.32±50.91) pg/ml) was increased significantly since 24 h in HIBD group(P＜0.05).Conclusion NLRP3 and its downstream inflammatory cytokines IL-1 β and IL-18 are up-regulated when HIBD occurs.The change of NLRP3protein expression in group HIBD is earlier than changes of neuron.NLRP3 signal may mediate and participate in the occurrence and development of HIBD.
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BACKGROUND:Very smal embryonic-like stem cells are a kind of non-hemopoietic stem cells, which have similar biological characteristics to embryonic stem cells. But the method of its culture and in vitro proliferation is rarely reported. Studies have speculated that human embryonic fibroblasts can provide a good microenvironment for in vitro culture and proliferation of very smal embryonic-like stem cells. OBJECTIVE:To isolate and cultivate human embryonic fibroblasts derived from human embryonic trunks and to establish a feeder layer culture system of human embryonic fibroblasts for culturing very smal embryonic-like stem cells derived from human bone marrow. METHODS:The human embryonic fibroblasts were isolated from the subcutaneous connective tissue of human embryos at pregnant 5-9 weeks using trypsin digestion method. Different concentrations of mitomycin C were used to pretreat feeder layers, which were used for cultivating very smal embryonic-like stem cells derived from human bone marrow. The effects of human embryonic fibroblasts and feeder layers were assessed by cel morphology and growth curves. RESULTS AND CONCLUSION:The human embryonic fibroblasts were successful y isolated and cultivated from human embryos, and they could be passaged beyond the 24th generation. The biologic characteristics of the cells had no changes after passage and cryopreservation. The optimal concentration of mytomcin C to inhibit proliferation of human embryonic fibroblasts was l2 mg/L for 3 hours. The human embryonic fibroblasts derived from human embryos are successful y isolated and cultivated and to produce feeder layers for very smal embryonic-like stem cells derived from human bone marrow.
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The establishment of clinical medicine with professional degree is an important reformation of high-level professionals in medical field. With the expansion of the number of postgraduates of clinical medicine with professional degree, how to establish and perfect the training mode and how to improve the training quality has become an important research for the postgraduate education with professional degree. This paper discusses the establishment and perfection of training mode of full-time postgraduates of clinical medicine with professional degree by combining the documents and development of professional degree in my university.
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By the survey of topic selection of medical master's dissertation for candidates with the same educational level,the writers think that the decided topics should be in close relation to their respective subjects and the operational and technical conditions of their institutes and the training institutes should be taken into full consideration.The decided topics should enhance theoretical and practical values and will be achieved in the required time as well.